CD105 Antibody

CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. CD105 is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P17813

Host

Mouse

Reactivity

Human

Immunogen

A partial recombinant protein corresponding to amino acids 74-251 of human CD105/Endoglin was used as the immunogen for the CD105 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ENG/3269

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD105 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD105 antibody is available for research use only.

Storage Conditions

Store the CD105 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD105 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human tonsil with CD105 antibody (clone ENG/3269) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.