Recombinant TAG-72 Antibody

This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

Host

Mouse

Reactivity

Human

Immunogen

The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this recombinant TAG-72 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RB72.3

Type

Recombinant

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant TAG-72 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant TAG-72 antibody is available for research use only.

Storage Conditions

Store the recombinant TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant TAG-72 antibody should be determined by the researcher.

Location

Cytoplasmic and cell surface

Image Legend

IHC staining of human breast carcinoma with recombinant TAG-72 antibody (clone rB72.3) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.