DNMT1 Antibody

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P26358

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 767-912 from the human protein was used as the immunogen for the DNMT1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

DNMT1/2061

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the DNMT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This DNMT1 antibody is available for research use only.

Storage Conditions

Store the DNMT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the DNMT1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human tonsil with DNMT1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.