Cathepsin D Antibody

Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins. It is synthesized as an inactive 52kDa preprocathepsin D that is cleaved and glycosylated to form a 48kDa procathepsin D and then further cleaved to produce 34kDa and 14kDa subunits (heavy and light chains, respectively) . Cathepsin D exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P07339

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 104-250) was used as the immunogen of the Cathepsin D antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CTSD/3082

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Cathepsin D antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cathepsin D antibody is available for research use only.

Storage Conditions

Store the Cathepsin D antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Cathepsin D antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human liver carcinoma + macrophages with Cathepsin D antibody (clone CTSD/3082) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.