CD35 Antibody / CR1

Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1) /CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, , Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P17927

Host

Mouse

Reactivity

Human

Immunogen

Follicular dendritic cells (FDC) from the tonsil were used as the immunogen for the CD35 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

To5

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD35 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD35 antibody is available for research use only.

Storage Conditions

Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD35 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

IHC staining of FFPE human tonsil with CD35 antibody (clone To5) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.