CPA1 Antibody / Carboxypeptidase A1

Human pancreatic procarboxypeptidase A exists as three different active forms, two of which are designated carboxypeptidase A1 (CPA1) and carboxypeptidase A2 (CPA2) . CPA1, also known as CPA, is a 419 amino acid secreted monomeric protein that is highly expressed in pancreatic tissue. Functioning as a pancreatic exopeptidase, CPA1 uses zinc as a cofactor to catalyze the release of C-terminal amino acids from a variety of proteins, thereby playing a key role in protein digestion and degradation. Via its catalytic activity, CPA1 is also thought to be involved in zymogen (proenzyme) inhibition, probably functioning to block enzyme activation pathways. Abnormal levels of CPA1 are associated with pancreatic cancer, suggesting a possible role in either tumor progression or tumor suppression events.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

P15085

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human Carboxypeptidase A1 protein was used as the immunogen for the CPA1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CPA1/2711

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Carboxypeptidase A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Carboxypeptidase A1 antibody is available for research use only.

Storage Conditions

Store the Carboxypeptidase A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Carboxypeptidase A1 antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

IHC staining of FFPE human pancreas with CPA1 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.