Collagen VII antibody / COL7A1

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q02388

Host

Mouse

Reactivity

Human

Immunogen

A recombinant full-length human Collagen VII protein was used as the immunogen for this Collagen VII antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

LH7.2

Applications

ELISA, WB, FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Collagen VII antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Collagen VII antibody is available for research use only.

Storage Conditions

Store the Collagen VII antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Collagen VII antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human skin with Collagen VII antibody (clone LH7.2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.