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Recombinant Adipophilin Antibody / Perilin 2 / ADRP / PLIN2

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q99541

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids 249-376) was used as the immunogen for this Adipophilin antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG1 k

Clone

RADFP/1493

Type

Recombinant

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant Adipophilin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Adipophilin antibody is available for research use only.

Storage Conditions

Store the recombinant Adipophilin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant Adipophilin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human adrenal tissue with recombinant Adipophilin antibody (clone rADFP/1493) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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