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Adipophilin Antibody

Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Product Specifications

Specifications

ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL

UniProt

Q99541

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids 249-376) was used as the immunogen for this Adipophilin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ADFP/1365

Applications

ELISA, FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Adipophilin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Adipophilin antibody is available for research use only.

Storage Conditions

Store the Adipophilin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Product Datasheet

https://www.nsjbio.com/tds-pdf/adipophilin-antibody-adfp1365-v7686

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Adipophilin antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Cytoplasmic

Image Legend

Flow cytometry testing of permeabilized human PMBC with Adipophilin antibody (clone ADFP/1365) ; Red=isotype control, Blue= Adipophilin antibody.

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