Adipophilin Antibody
Recognizes a protein of 48kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL
UniProt
Q99541
Host
Mouse
Reactivity
Human
Immunogen
A human recombinant partial protein (amino acids 249-376) was used as the immunogen for this Adipophilin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ADFP/1365
Applications
ELISA, FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Adipophilin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Adipophilin antibody is available for research use only.
Storage Conditions
Store the Adipophilin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Adipophilin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Flow cytometry testing of permeabilized human PMBC with Adipophilin antibody (clone ADFP/1365) ; Red=isotype control, Blue= Adipophilin antibody.
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