Clathrin Heavy Chain Antibody

Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1-2 µg/mL

UniProt

Q00610, P53675

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human Clathrin Heavy Chain protein was used as the immunogen for this Clathrin Heavy Chain antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CHC/1432

Applications

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Clathrin Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Clathrin Heavy Chain antibody is available for research use only.

Storage Conditions

Store the Clathrin Heavy Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Immunofluorescent staining of permeabilized human HeLa cells with Clathrin Heavy Chain antibody (clone CHC/1432, green) and Reddot nuclear stain (red) .