CKB Antibody / Creatine Phosphokinase-BB

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type) . There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL

UniProt

P12277

Host

Mouse

Reactivity

Human, Mouse, Rat, Hamster, Guinea pig

Immunogen

Recombinant human full-length protein was used as the immunogen for the CKB antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CPTC-CKB-2

Applications

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CKB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CKB antibody is available for research use only.

Storage Conditions

Store the CKB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CKB antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using Creatine kinase B antibody (clone CPTC-CKB-2) . Predicted molecular weight ~43 kDa.