CHD4 Antibody / Chromodomain helicase DNA binding protein 4
CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q14839
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Amino acids ASGLGSPSPCSAGSEEEDM and CSRLANRAPEPPPQQVAQQQ were used as the immunogen for the CHD4 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3F2/4
Applications
ELISA, WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CHD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CHD4 antibody is available for research use only.
Storage Conditions
Store the CHD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CHD4 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of permeabilized human MCF7 cells with CHD4 antibody (clone 3F2/4, green) and Phalloidin (red) .
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