UPK1A Antibody / Uroplakin 1A

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O00322

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 114-173 were used as the immunogen for the UPK1A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

UPK1A/2922

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the UPK1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This UPK1A antibody is available for research use only.

Storage Conditions

Store the UPK1A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the UPK1A antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human urinary bladder with UPK1A antibody (clone UPK1A/2922) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.