CDX2 Antibody

The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinomas and carcinoidsand is especially useful to distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma.However, mucinous carcinomas of the ovary also express CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous carcinoma of the ovary.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q99626

Host

Mouse

Reactivity

Human

Immunogen

A full length recombinant human protein was used as the immunogen for the CDX2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CDX2/2214

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CDX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDX2 antibody is available for research use only.

Storage Conditions

Store the CDX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CDX2 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human colon carcinoma with CDX2 antibody (clone CDX2/2214) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling prior to testing.