TUBB3 Antibody / Beta Tubulin

This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3.In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

UniProt

Q13509

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 437-450 were used as the immunogen for the TUBB3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

TUBB3/3731

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the TUBB3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TUBB3 antibody is available for research use only.

Storage Conditions

Store the TUBB3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the TUBB3 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human brain with TUBB3 antibody (TUBB3/3731) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.