CDK2 Antibody
In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells in 0.1ml
UniProt
P24941
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Recombinant human CDK2 was used as the immunogen for this CDK2 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AN4.3
Applications
WB, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CDK2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CDK2 antibody is available for research use only.
Storage Conditions
Store the CDK2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CDK2 antibody should be determined by the researcher.
Image Legend
SDS-PAGE analysis of purified, BSA-free CDK2 antibody (clone AN4.3) as confirmation of integrity and purity.
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