CDH16 Antibody / Cadherin 16

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin (also called CDH16 and Cadherin 16) . Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order BSA-free format for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

O75309

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 371-507) was used as the immunogen for the CDH16 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

CDH16/2448

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CDH16 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDH16 antibody is available for research use only.

Storage Conditions

Store the CDH16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CDH16 antibody should be determined by the researcher.

Location

Cell surface with some cytoplasmic

Image Legend

IHC testing of FFPE human renal cell carcinoma and Cadherin 16 antibody. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.