Recombinant SOX2 Antibody

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity) . Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity) . Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P48431

Host

Rabbit

Reactivity

Human

Immunogen

Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

SBX2-1R

Type

Recombinant

Applications

WB, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the recombinant SOX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant SOX2 antibody is available for research use only.

Storage Conditions

Store the recombinant SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SOX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC staining of FFPE human cervical carcinoma with recombinant SOX2 antibody (clone SBX2-1R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.