Granzyme B Antibody

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P10144

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 73-187 were used as the immunogen for the Granzyme B antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

GZB-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Granzyme B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Granzyme B antibody is available for research use only.

Storage Conditions

Store the Granzyme B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Granzyme B antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human spleen with Granzyme B antibody (clone GZB-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.