ATG5 Antibody

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

Q9H1Y0

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein (amino acids 1-119) was used as the immunogen for the ATG5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ATG5/2492

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the ATG5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ATG5 antibody is available for research use only.

Storage Conditions

Store the ATG5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the ATG5 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human colon carcinoma with ATG5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.