Prolactin Antibody / PRL

Prolactin is a growth factor that is secreted by the anterior pituitary. It is necessary for the proliferation and differentiation of the mammary glands. Prolactin is useful in the classification of pituitary tumors and study of pituitary disease. It also plays a role in the development of mammary cancer, functioning dually as a mitogen and a differentiating agent.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01236

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids 63-201 was used as the immunogen for the Prolactin antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PRL/2642

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Prolactin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Prolactin antibody is available for research use only.

Storage Conditions

Store the Prolactin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Prolactin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human pituitary tissue with Prolactin antibody (clone PRL/2642) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.