PAPP-A Antibody / Pappalysin-1

Pregnancy Associated Plasma Protein (PAPP-A) is found in maternal blood that increases as pregnancy progresses, although it is not specific to pregnancy. It is principally expressed in the syncytiotrophoblast of the placenta, which forms the main source of circulating maternal PAPP-A. It cleaves insulin-like growth factor binding proteins (IGFBPs), IGFBP-4 and IGFBP-5. IGFBP-4 cleavage is enhanced significantly in the presence of bound IGF, whereas IGFBP-5 cleavage is inhibited slightly by IGF presence. It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses. PAPPA has also been suggested as a potential biomarker of acute myocardial infarction and Coronary Artery Disease (CAD) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q13219

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 351-523 from the human protein was used as the immunogen for the PAPP-A antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PAPPA/2715

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PAPP-A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PAPP-A antibody is available for research use only.

Storage Conditions

Store the PAPP-A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human placenta with PAPP-A antibody (clone PAPPA/2715) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.