NPM Antibody / Nucleophosmin

The protein encoded by the NPM1 gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P06748

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 185-287 from the human protein was used as the immunogen for the NPM antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NLPP1-1

Applications

IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the NPM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This NPM antibody is available for research use only.

Storage Conditions

Store the NPM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human basal cell carcinoma with NPM antibody (clone NLPP1-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.