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PRL Antibody / Prolactin

The PRL gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P01236

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids 63-201 was used as the immunogen for the PRL antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PLCN-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PRL antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PRL antibody is available for research use only.

Storage Conditions

Store the PRL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the PRL antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Image Legend

IHC testing of FFPE human pituitary tissue with PRL antibody (clone PLCN-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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