PHB Antibody / Prohibitin

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Ant-iproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P35232

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the PHB antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

PBTN-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the PHB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PHB antibody is available for research use only.

Storage Conditions

Store the PHB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human liver tissue with PHB antibody (clone PBTN-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.