MSH6 Antibody

MSH6 is a component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P52701

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

MST6-2

Applications

FACS, IF, IHC-P, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MSH6 antibody is available for research use only.

Storage Conditions

Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nucleus

Image Legend

IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MST6-2) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.