CK5 Antibody / KRT5 / Cytokeratin 5

The protein encoded by the KRT5 gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P13647

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human Cytokeratin 5 protein fragment within amino acids 316-590 was used as the immunogen for the CK5 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTKN5-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the CK5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CK5 antibody is available for research use only.

Storage Conditions

Store the CK5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with CK5 antibody (clone CTKN5-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.