TIGIT Antibody

TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is a immuno-oncology target for therapy.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

Q495A1

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 22-141 from the human protein was used as the immunogen for the TIGIT antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TIGIT/3106

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the TIGIT antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This TIGIT antibody is available for research use only.

Storage Conditions

Store the TIGIT antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the TIGIT antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with biotinylated TIGIT antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.