TIGIT Antibody / VSTM3 / VSIG9

TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is a immuno-oncology target for therapy.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q495A1

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human TIGIT protein fragment within amino acids 194-244 was used as the immunogen for the TIGIT antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

TIGIT/3106

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TIGIT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TIGIT antibody is available for research use only.

Storage Conditions

Store the TIGIT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Image Legend

IHC staining of FFPE human prostate carcinoma with TIGIT antibody (clone TIGIT/3016) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.