TIGIT Antibody / VSTM3 / VSIG9

TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is an immuno-oncology target for therapy.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q495A1

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human TIGIT protein fragment within amino acids 22-141 was used as the immunogen for the TIGIT antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

TIGIT/3017

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the TIGIT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TIGIT antibody is available for research use only.

Storage Conditions

Store the TIGIT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Applications Notes

Optimal dilution of the TIGIT antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human prostate carcinoma with TIGIT antibody (clone TIGIT/3017) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.