ICOSLG Antibody / ICOS Ligand / B7RP-1

This MAb recognizes a protein of 36kDa, identified as ICOSLG, which is also known as B7-related protein-1 (B7RP-1) .T-cell co-stimulatory molecule, inducible co-stimulator (ICOS) /B7-related protein-1 (B7RP-1, B7-H2, GL50, ICOS-L) is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNFÎ±), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

O75144

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment of human ICOS Ligand / B7RP-1 protein within amino acids 23-149 was used as the immunogen for the ICOSLG antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ICOSL/3111

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the ICOSLG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ICOSLG antibody is available for research use only.

Storage Conditions

Store the ICOSLG antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cell surface

Image Legend

IHC staining of FFPE human prostate carcinoma with ICOSLG antibody (clone ICOSL/3111) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.