Mucin 4 Antibody / MUC4

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q99102

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 1730-1864 from the human protein were used as the immunogen for this Mucin 4 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MUCN4-1

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Mucin 4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Mucin 4 antibody is available for research use only.

Storage Conditions

Store the Mucin 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma with Mucin 4 antibody (clone MUCN4-1) . HIER: requires steaming of sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.