Recombinant CD3e Antibody
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P07766
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 23-119) was used as the immunogen for the recombinant CD3e antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
C3e/3125R
Type
Recombinant
Applications
ELISA, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD3e antibody is available for research use only.
Storage Conditions
Store the recombinant CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of human MOLT-4 cell lysate with recombinant CD3e antibody (clone C3e/3125R) . Predicted molecular weight ~23 kDa.
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