Recombinant CD3e Antibody
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
CAS Number
9000-83-3
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P07766
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 23-119) was used as the immunogen for the recombinant CD3e antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
C3e/3125R
Type
Recombinant
Applications
ELISA, WB, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD3e antibody is available for research use only.
Storage Conditions
Store the recombinant CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD3e antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of human MOLT-4 cell lysate with recombinant CD3e antibody (clone C3e/3125R) . Predicted molecular weight ~23 kDa.
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