Nucleophosmin Antibody / NPM1

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM) . It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P06748

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

NPM1/3286

Applications

IF, ELISA, WB, IHC-P, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Nucleophosmin antibody is available for research use only.

Storage Conditions

Store the Nucleophosmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear, cytoplasmic

Image Legend

IHC staining of FFPE human basal cell carcinoma with Nucleophosmin antibody (clone NPM1/3286) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.