Nucleophosmin Antibody / NPM1
Recognizes a 33-38kDa glycoprotein, identified as Nucleophosmin (NPM) . It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
Product Specifications
CAS Number
9007-83-4
Specifications
UniProt
P06748
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
NPM1/3287
Applications
ELISA, IHC-P, WB, IF, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Nucleophosmin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nucleophosmin antibody is available for research use only.
Storage Conditions
Store the Nucleophosmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear, cytoplasmic
Image Legend
Western blot testing of human K562 cell lysate with Nucleophosmin antibody (clone NPM1/3287) . Expected molecular weight: ~38 kDa.
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