Calbindin 2 Antibody / CALB2 / Calretinin

It recognizes a protein of about 29kDa, which is identified as Calretinin (also known as Calbindin 2) . Calretinin is a vitamin D-dependent calcium-binding protein involved in calcium signaling. It is present in subsets of neurons throughout the brain and spinal chord, including sensory ganglia. Antibody to calretinin is useful in differentiating mesothelioma from adenocarcinomas of the lung. It also aids in differentiating adrenal cortical neoplasms from pheochromocytomas.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P22676

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 23-242 from the human protein was used as the immunogen for the Calretinin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CALB2/2786

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the Calbindin 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Calbindin 2 antibody is available for research use only.

Storage Conditions

Store the Calbindin 2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Calbindin 2 antibody should be determined by the researcher.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human mesothelioma with Calbindin 2 antibody (clone CALB2/2786) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.