Sarcomeric Alpha Actinin Antibody / ACTN2
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P35609
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 557-692 from the human protein were used as the immunogen for this Sarcomeric Alpha Actinin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ACTN2/3293
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.05% sodium azide
Reconstitution
Store the Sarcomeric Alpha Actinin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Sarcomeric Alpha Actinin antibody is available for research use only.
Storage Conditions
Store the Sarcomeric Alpha Actinin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic
Image Legend
Western blot testing of human skeletal muscle lysate with Sarcomeric Alpha Actinin antibody (clone ACTN2/3293) . Predicted molecular weigth ~103 kDa.
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