CD47 Antibody

This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 2-4 µg/mL, Flow cytometry: 5ul per million cells, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

Q08722

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 18-135 from the human protein was used as the immunogen for the CD47 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CD47/3019

Applications

IF, FACS, IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD47 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This CD47 antibody is available for research use only.

Storage Conditions

Store the CD47 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD47 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human prostate carcinoma with biotinylated CD47 antibody (clone CD47/3019) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.