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ACTN2 Antibody / Sarcomeric Alpha Actinin / Alpha Actinin 2

The ACTN2 gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P35609

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 557-692 from the human protein were used as the immunogen for this ACTN2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SAMA-1

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.05% sodium azide

Reconstitution

Store the ACTN2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ACTN2 antibody is available for research use only.

Storage Conditions

Store the ACTN2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human skeletal muscle tissue with Sarcomeric Alpha Actinin antibody (clone SAMA-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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