Annexin A1 Antibody
The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P04083
Host
Mouse
Reactivity
Human
Immunogen
A mixture of native proteins from human bone tissues was used as the immunogen for the Annexin A1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
5E4/1
Applications
ELISA, FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Annexin A1 antibody is available for research use only.
Storage Conditions
Store the Annexin A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Annexin A1 antibody to be determined by the researcher.
Location
Nuclear and Cytoplasmic
Image Legend
Flow testing of fixed and permeabilized human HeLa cells with Annexin A1 antibody (clone 5E4/1) . Red=isotype control, Blue= Annexin A1 antibody.
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