Calcineurin Antibody / PPP3CA

Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Product Specifications

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

Q08209

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the Calcineurin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CALNA/2353

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Calcineurin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Calcineurin antibody is available for research use only.

Storage Conditions

Store the Calcineurin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic, cell surface, nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma with Calcineurin antibody (clone CALNA/2353) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.