Calcineurin Antibody / PPP3CA

Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q08209

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant human protein was used as the immunogen for the Calcineurin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CALNA/2353

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Calcineurin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Calcineurin antibody is available for research use only.

Storage Conditions

Store the Calcineurin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, cell surface, nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma with Calcineurin antibody (clone CALNA/2353) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.