Recombinant CD81 Antibody

Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1) . CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Product Specifications

CAS Number

9000-83-3

Specifications

ELISA: order BSA/sodium azide-free format for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 0.5-1ug/million cells

UniProt

P60033

Host

Rabbit

Reactivity

Human

Immunogen

A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the recombinant CD81 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

C81/2885R

Type

Recombinant

Applications

ELISA, IHC-P, FACS

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD81 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD81 antibody is available for research use only.

Storage Conditions

Store the recombinant CD81 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cytoplasmic, membranous

Image Legend

Flow cytometry analysis of bead-bound exosomes derived from MCF-7 cells using recombinant CD81 antibody (clone C81/2885R) . Gray=unstained cells, Orange = recombinant CD81 antibody.