Recombinant CD44 Antibody

Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P16070

Host

Rabbit

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for the recombinant CD44 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

HCAM/2875R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD44 antibody is available for research use only.

Storage Conditions

Store the recombinant CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of human MCF7 cell lysate with recombinant CD44 antibody (clone HCAM/2875R) . Predicted molecular weight ~81 kDa but may be observed at higher molecular weights due to glycosylation.