GATA3 Antibody

GATA3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA3 expression is primarily seen in breast and urothelial carcinomas. Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Western blot: 0.5-1 µg/mL

UniProt

P23771

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 357-463 from the human protein were used as the immunogen for this GATA3 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

GATA3/2446

Applications

ELISA, FACS, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the GATA3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This GATA3 antibody is available for research use only.

Storage Conditions

Store the GATA3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Nuclear

Image Legend

IHC testing of FFPE human breast carcinoma with GATA3 antibody. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool prior to testing.