Recombinant Mitochondrial Marker Antibody

This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Unknown

Host

Rabbit

Reactivity

Human

Immunogen

A semi-purified mitochondria preparation was used as the immunogen for this recombinant Mitochondrial Marker antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

MTC02/2860R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the recombinant Mitochondrial Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant Mitochondrial Marker antibody is available for research use only.

Storage Conditions

Store the recombinant Mitochondrial Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Location

Mitochondria in cytoplasm

Image Legend

IHC staining of FFPE human pancreas with recombinant Mitochondrial Marker antibody (clone MTC02/2860R) . HIER: boil tissue sections in 1mM EDTA buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT prior to testing.