Recombinant CD56 Antibody

Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Flow cytometry: 1-2ug/million cells

UniProt

P13591

Host

Rabbit

Reactivity

Human

Immunogen

A full length recombinant human protein was used as the immunogen for this recombinant CD56 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

NCAM1/2217R

Type

Recombinant

Applications

IHC-P, FACS

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant CD56 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant CD56 antibody is available for research use only.

Storage Conditions

Store the recombinant CD56 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface, cytoplasmic

Image Legend

Flow cytometry staining of lymphocyte-gated human PBM cells with recombinant CD56 antibody (clone NCAM1/2217R) ; Gray=unstained cells, Green= CD56 antibody stained cells.