Recombinant NGFR Antibody / p75NTR

CD271/NGFR pays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity) . Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

UniProt

P08138

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 281-421 from the human protein was used as the immunogen for the recombinant NGFR antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CDLA271-2R

Type

Recombinant

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the recombinant NGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This recombinant NGFR antibody is available for research use only.

Storage Conditions

Store the recombinant NGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Location

Cell surface and cytoplasmic

Image Legend

IHC testing of FFPE human melanoma with recombinant NGFR antibody (clone CDLA271-2R) . Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool prior to testing.